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Pyrrolidinedithiocarbamate ammonium

产品编号 T3147Cas号 5108-96-3
别名 PDTC, Pyrrolidinedithiocarbamate, 吡咯烷二硫代氨基甲酸铵, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, Ammonium pyrrolidinedithiocarbamate, 吡咯烷二硫代甲酸铵

Pyrrolidinedithiocarbamate ammonium (1-Pyrrolidinedithiocarboxylic acid ammonium salt) 是一种选择性的、能透过血脑屏障的NF-κB 抑制剂。

Pyrrolidinedithiocarbamate ammonium

Pyrrolidinedithiocarbamate ammonium

纯度: ≥98%
产品编号 T3147 别名 PDTC, Pyrrolidinedithiocarbamate, 吡咯烷二硫代氨基甲酸铵, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, Ammonium pyrrolidinedithiocarbamate, 吡咯烷二硫代甲酸铵Cas号 5108-96-3

Pyrrolidinedithiocarbamate ammonium (1-Pyrrolidinedithiocarboxylic acid ammonium salt) 是一种选择性的、能透过血脑屏障的NF-κB 抑制剂。

规格价格库存数量
100 mg¥ 286现货
200 mg¥ 397现货
500 mg¥ 672现货
1 mL x 10 mM (in DMSO)¥ 185现货
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纯度:≥98%
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产品介绍

生物活性
产品描述
Pyrrolidinedithiocarbamate ammonium (1-Pyrrolidinedithiocarboxylic acid ammonium salt), a selective NF-κB inhibitor, inhibits translation of nitric oxide synthase mRNA to prevent induction.
体外活性
细胞预处理PDTC(3-1000 mM)剂量依赖性地减少IL-8产生。此外,PDTC(100 mM)抑制IL-8 mRNA的积累。PDTC通过抑制NF-kB DNA结合及其依赖的转录活性,从而阻止NF-kB的激活。综合我们的数据表明,通过PDTC抑制NF-kB可以降低肠上皮细胞IL-8的产生[1]。
体内活性
DSS+PDTC处理的第二组表现出抑制小肠长度缩短和DAI得分降低。该组中活化的NF-κB水平以及IL-1β和TNF-α水平显著低于对照组。这些发现表明,通过PDTC抑制NF-κB活性可以延缓由炎症引起的粘膜组织缺陷(侵蚀或溃疡)的愈合,但它能强烈抑制炎症细胞因子(IL-1β和TNF-α)的表达,从而显著缓解结肠炎。PDTC对于治疗溃疡性结肠炎具有重要价值[2]。
激酶实验
All binding studies are performed in an HTRF assay buffer consisting of dPBS supplemented with 0.1% (with v) bovine serum albumin and 0.05% (v/v) Tween-20. For the PD-l-Ig/PD-Ll-His binding assay, inhibitors are pre-incubated with PD-Ll-His (10 nM final) for 15 m in 4 μL of assay buffer, followed by addition of PD-l-Ig (20 nM final) in 1 μL of assay buffer and further incubation for 15 m. PD-L1 from either human, cyno, or mouse are used. HTRF detection is achieved using europium crypate-labeled anti- Ig (1 nM final) and allophycocyanin (APC) labeled anti-His (20 nM final). Antibodies are diluted in HTRF detection buffer and 5 μL is dispensed on top of binding reaction. The reaction mixture is allowed to equilibrate for 30 minutes and signal (665 nm/620 nm ratio) is obtained using an En Vision fluorometer. Additional binding assays are established between PD-1-Ig/PD-L2-His (20, 5 nM, respectively), CD80-His/PD-Ll-Ig (100, 10 nM, respectively) and CD80-His/CTLA4-Ig (10, 5 nM, respectively).
细胞实验
The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy's 5A medium supplemented with 10% fetal bovine serum. To study the effect of PDTC on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1b for 18 h. Various concentrations (3-1000 mM) of PDTC or its vehicle (culture medium) are added to the cells 30 min prior to IL-1b stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay, as described previously employing the multiple antibody sandwich principle that specifically detects human IL-8[1].
别名PDTC, Pyrrolidinedithiocarbamate, 吡咯烷二硫代氨基甲酸铵, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, Ammonium pyrrolidinedithiocarbamate, 吡咯烷二硫代甲酸铵
化学信息
分子量164.29
分子式C5H12N2S2
CAS No.5108-96-3
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
DMSO: 75 mg/mL (456.51 mM)
5% DMSO+95% Saline: 0.6 mg/mL (3.65 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
溶液配制表
5% DMSO+95% Saline/DMSO
1mg5mg10mg50mg
1 mM6.0868 mL30.4340 mL60.8680 mL304.3399 mL
DMSO
1mg5mg10mg50mg
5 mM1.2174 mL6.0868 mL12.1736 mL60.8680 mL
10 mM0.6087 mL3.0434 mL6.0868 mL30.4340 mL
20 mM0.3043 mL1.5217 mL3.0434 mL15.2170 mL
50 mM0.1217 mL0.6087 mL1.2174 mL6.0868 mL
100 mM0.0609 mL0.3043 mL0.6087 mL3.0434 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

对于不同动物的给药剂量换算,您也可以参考 更多

关键词

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